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第1章：亚甲基四氢叶酸还原酶（MTHFR）多态性与非酒精性脂肪性肝病（NAFLD）之间的关联风险：meta分析 发布时间：2016年4月29日http://dx./10.1371/journal.pone.0154337 ###FS的宗旨：科学自由分享、人人平等，共求真理### Inclusion and exclusion criteria The eligible case-control studies were identified according to the following inclusion and exclusion criteria. Inclusion criteria: 1) The data on the association between MTHFR polymorphisms and susceptibility to NAFLD was provided; 2) The individual genotype frequencies for MTHFR polymorphisms could be extracted. Exclusion criteria: 1) duplicated studies; 2) reviews or books; 3) non-clinical data; 4) other genes; 5) non-NAFLD diseases; 6) case, trial, or non-polymorphism; 7) meeting/conference abstracts; 8) unavailable data. Data extraction strategy Data was extracted from qualified articles independently by the authors (MYS LZ SLS JNL) using the same reporting form. The controversial evaluations were resolved through discussion. If the data was unavailable, an attempt was made to contact corresponding author to request missing data via E-mail. The following information was extracted: mutation site, first author, year of publication, country, ethnicity, sample sizes in case and control group, source of control, genotyping method, gender and age in case group, disease group, allele and genotype frequencies in each group, The Χ2 and P value of Hardy-Weinberg Equilibrium (HWE) test in control group. HWE value was calculated by chi-squared test and P value less than 0.05 was considered a departure from HWE. Statistical analysis The P value, odd radio (OR) and corresponding 95% confidence interval (CI) were calculated by Mantel-Haenszel statistics under the allele, homozygote, heterozygote, dominant or recessive models. P value <0.05 was="" considered="" statistically="" significant="" association="" between="" c677t="" and="" a1298c="" polymorphisms="" of="" mthfr="" and="" nafld="" risks.="" χ2-based="" q="" statistic="" and="" i2test="" were="" applied="" to="" analyze="" the="" overall="" heterogeneities.=""> When I2 values < 25%="" or="" p="" value="" of="" heterogeneity="">0.10, a fixed-effect model was selected for Mantel-Haenszel statistics. Otherwise, a random-effect model was used [32–35]. When significant heterogeneity existed, sensitivity analysis was also performed to analyze the study that influenced homogeneity of the included studies. The potential publication bias was evaluated by Begg’s funnel plot with pseudo 95% confidence limits [36]. Statistical analyses were conducted by Review Manager Version 5.0 (The Nordic Cochrane Centre, The Cochrane Collaboration, Denmark) and Stata/SE 12.0 (StataCorp, College Station, USA) software. 纳入标准（正文） 根据以下纳入和排除标准确定合格的病例对照研究。 纳入标准：1）提供MTHFR多态性与NAFLD易感性之间关系的数据; 2）可以提取MTHFR多态性的个体基因型频率。 排除标准：1）重复研究; 2）评论或书籍; 3）非临床资料; 4）其他基因; 5）非NAFLD疾病; 6）病例、试验或非多态性; 7）会议/会议摘要; 8）不可用数据。 数据提取策略 使用相同的报告表格，作者独立地从合格的文章中提取数据。有争议的评估通过讨论得到解决。如果数据不可用，则尝试联系相应的作者以通过电子邮件请求丢失的数据。 提取以下信息：突变位点，第一作者，出版年份，国家，种族，病例和对照组的样本量，对照来源，基因分型方法，病例组，疾病组，等位基因和基因型频率中的性别和年龄各组，对照组的Hardy-Weinberg平衡（HWE）检验的X2和P值。 HWE值通过卡方检验计算，P值小于0.05被认为是偏离HWE。 统计分析 通过等位基因，纯合子，杂合子，显性或隐性模型下的Mantel-Haenszel统计量计算P值，OR和相应的95％置信区间（CI）。 P值<> 当I2值<25％或异质性p值> 0.10时，选择Mantel-Haenszel统计量的固定效应模型。否则，随机效应模型用于[ 32 - 35 ]。 当存在显着的异质性时，还进行敏感性分析以分析影响所包含研究的同质性的研究。通过Begg的漏斗图评估潜在的出版偏倚，具有95％的置信限度[ 36 ]。 统计分析由Review Manager 5.0版（Nordic Cochrane Center，The Cochrane Collaboration，Denmark）和Stata / SE 12.0（StataCorp，College Station，USA）软件进行。 ###猴哥摘要解析：
2.“如果数据不可用，则尝试联系相应的作者以通过电子邮件请求丢失的数据。”审稿人会要求提供邀请的原始数据的邮件。 您好！非常冒昧打搅您了。 猴哥之前的推文有讲： 4 数据提取： 5.本文提供了一个非常好的模板 猴哥：Freescience公众号meta分析栏目现任主编。副主任医师，武汉大学肿瘤学博士，专注于胃肠道肿瘤分子生物学机制、系统评价/Meta分析、数据挖掘、临床统计研究。 |
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来自： chengxiaoli115 > 《总论》
