Output
iSeq100?SequencingSystem
1×36bp
1×50bp
1×75bp
2×150bp
4M
1.2Gb
>85%
>75%
10hours
17.5hours
MaximumReadsperRun
SequencingReagentKits
Reads(PassingFilter)/Run
RunConfiguration
RunTimec
144Mb
9hours
174–200k/mm2
200Mb
300Mb
>80%
2×75bp
600Mb
13hours
1.2Gb
MiniSeqSystem
MiniSeqSystemHigh-OutputKit
6.6–7.5Gb
~24hrs
129-165K/mm2
3.3–3.75Gb
13hrs
1.65–1.875Gb
7hrs
MiniSeqSystemMid-OutputKit
2.1–2.4Gb
17hrs
MiSeqReagentKitv2
540–610Mb
>90%
~4hours
865-965k/mm2
750–850Mb
>90%
~5.5hours
4.5–5.1Gb
>80%
~24hours
7.5–8.5Gb
>75%
~39hours
MiSeqReagentKitv3
3.3–3.8Gb
>85%
~21hours
1200-1400k/mm2
13.2–15Gb
>70%
~56hours
MiSeqReagentKitv2Micro
~19hours
MiSeqReagentKitv2Nano
300Mb
~17hours
500Mb
~28hours
NextSeqSeriesHigh-OutputKit
25–30Gb
11hours
50–60Gb
18hours
100–120Gb
29hours
NextSeqSeriesMid-OutputKit
16.25–19.5Gb
15hours
32.5–39Gb
26hours
HiSeq?2500SequencingSystem
1×36
DualFlowCell128–144Gb
SingleFlowCell64–72Gb
29hours
2×50
DualFlowCell360–400Gb
SingleFlowCell180–200Gb
2.5days
2×100
DualFlowCell720–800Gb
SingleFlowCell360–400Gb
5days
DualFlowCell900Gb–1Tb
SingleFlowCell450–500Gb
6days
400M
800M
130M
260M
QualityScoresb
baseshigherthanQ30
NextSeq?550SequencingSystem
DualFlowCell18–22Gb
SingleFlowCell9–11Gb
SingleFlowCell25–30Gb
DualFlowCell50–60Gb
DualFlowCell100–120Gb
SingleFlowCell50–60Gb
DualFlowCell150–180Gb
SingleFlowCell75–90Gb
DualFlowCell250–300Gb
SingleFlowCell125–150Gb
2×150
7hours
16hours
27hours
40hours
60hours
≥
85%
≥
85%
Instrument
NextSeq?550DxHigh-OutputReagentKitv2
>?75%
NextSeq?550Dx
MiSeqDxSystem
MiSeqSystem
MiSeqFGxSystem
MiSeqDxReagentKitv3
MiSeqFGxReagentKitTG–143–1001
ClusterDensity
桌面式测序仪
生产规模型测序仪
2×150bp
【
FC-420-1003
】
2×75bp
【
FC-420-1002
】
1×75bp
【
FC-420-1001
】
2×150bp
【
FC-420-1004
】
2×25bp
【
MS-102-2001
】
2×150bp
【
MS-102-2002
】
2×250bp
【
MS-102-2003
】
2×75bp
【
MS-102-3001
】
2×300bp
【
MS-102-3003
】
2×150bp
【
MS-103-1002
】
2×150bp
【
MS-103-1001
】
2×250bp
【
MS-103-1003
】
1×75bp
【
20024906
】
2×75bp
【
20024909
】
2×150bp
【
20024908
】
2×75bp
【
20024904
】
2×150bp
【
20024905
】
2?×?150?bp
【
20019554
】
≥
?90?Gb
≥
80%
2×125
【
FC–401–4003
】
2×100
【
FC–402–4021
】
≥
80%
≥
75%
2×250
【
FC–402–4023
】
iSeq100i1Reagent(300-cyclesinglekit)20021533
iSeq100i1Reagent4Pack(300-cyclequadkit)20021534
singlereadsUpto4million
paired-endUpto8million
105–125Gb
325–375Gb
650–750Gb
singlereads400million
paired-end800million
singlereads130million
paired-end260million
singlereads4million
paired-end8million
singlereads1million
paired-end2million
singlereads22–25million
paired-end44–50million
singlereads12–15million
paired-end24–30million
singlereads7–8million
paired-end14–16million
singlereads44–50million
paired-end22–25million
25M
50M
8M
16M
12–15M
24–30M
22–25M
44–50M
4M
8M
1M
2M
2.1–2.5billion
1300–1500Gb
210–250Gb
HiSeq3000System
HiSeq4000System
1310–1524?Kclusters/mm2
1–3.5days
≥
85%
≥
80%
≥
75%
HiSeq3000/4000SBSKit(50cycles)
HiSeq3000/4000SBSKit(150cycles)
HiSeq3000/4000SBSKit(300cycles)
1×50bp
【
FC-410-1001
】
2×75bp
【
FC-410-1002
】
2×150bp
【
FC-410-1003
】
4.3–5billion
1or2Flow
HighOutputKits
HiSeqSBSV4
DualFlowCell195–105Gb
SingleFlowCell47–52Gb
DualFlowCell1270–300Gb
SingleFlowCell135–150Gb
DualFlowCell1540–600Gb
SingleFlowCell270–300Gb
DualFlowCell1N/A
SingleFlowCellN/A
2
天
5.5
天
11
天
610–678K/mm2
870–930K/mm2
DualFlowCellUpto600million
SingleFlowCellUpto300million
HighOutputKits
TruSeqSBSV3
DualFlowCell
：
singlereadsUpto4billion
paired-endUpto8billion
SingleFlowCell
：
singlereadsUpto2billion
paired-endUpto4billion
DualFlowCell
：
singlereadsUpto30billion
paired-endUpto60billion
SingleFlowCell
：
singlereadsUpto15billion
paired-endUpto30billion
Rapid-RunMode
HiSeqRapidSBSKitV2
700–820K/mm2
HiSeqXTen
<3days
≥
75%
HiSeq?XTenReagentKitv2.5
2×150bp
【
FC-501-2501
】
HiSeq?XFive
HiSeq?XFiveReagentKitv2.5
Dualflowcell:1.6-1.8Tb
Dualflowcell:5.3-6billion
1255–1412Kclusters/mm2
SingleFlowCell
：
800-900Gb
SingleFlowCell
：
2.6-3.0billion
2
×
150bp
【
FC-502-2501
】
2x50bp
2x100bp
2x150bp
NovaSeq5000b
和
6000
系统
S1b:167Gb
S2:280-333Gb
S1b:333Gb
S2:560-667Gb
S1b:500Gb
S2:850-1000Gb
NovaSeq6000
S3b:2000Gb
S4b:3000Gb
S3b:NA
S4b:NA
≥
85%
≥
80%
≥
75%
≤
19hours
≤
29hours
≤
40hours
SingleFlowCell
：
S1b:1.6billion
S2:2.8-3.3billion
SingleFlowCell
：
S3b:6.6billion
S4b:10billion
NovaSeq5000/6000S2ReagentKit
(300cycles)
NovaSeq5000/6000S2ReagentKit(200cycles
NovaSeq5000/6000S2ReagentKit
(100cycles)
2x50bp
【
20012862
】
2x100bp
【
20012861
】
2x150bp
【
20012860
】
MiSeqDx
基因测序仪已经获得中国国家药品监督管理局（
CNDA
）的批准
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