|
Bacteroides thetaiotaomicron-Infecting Bacteriophage Isolates Inform Sequence-Based Host Range Predictions 多形拟杆菌噬菌体菌株提示可基于序列预测噬菌体宿主范围 10.1016/j.chom.2020.06.011 07-10, Article Abstract & Authors:展开 Abstract:收起
Our emerging view of the gut microbiome largely focuses on bacteria, while less is known about other microbial components, such as bacteriophages (phages). Though phages are abundant in the gut, very few phages have been isolated from this ecosystem. Here, we report the genomes of 27 phages from the United States and Bangladesh that infect the prevalent human gut bacterium Bacteroides thetaiotaomicron. These phages are mostly distinct from previously sequenced phages with the exception of two, which are crAss-like phages. We compare these isolates to existing human gut metagenomes, revealing similarities to previously inferred phages and additional unexplored phage diversity. Finally, we use host tropisms of these phages to identify alleles of phage structural genes associated with infectivity. This work provides a detailed view of the gut’s “viral dark matter” and a framework for future efforts to further integrate isolation- and sequencing-focused efforts to understand gut-resident phages.
First Authors:
Andrew J Hryckowian,Bryan D Merrill Correspondence Authors:
Andrew J Hryckowian,Justin L Sonnenburg All Authors:
Andrew J Hryckowian,Bryan D Merrill,Nathan T Porter,William Van Treuren,Eric J Nelson,Rebecca A Garlena,Daniel A Russell,Eric C Martens,Justin L Sonnenburg
|
