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本公众号每天分享一篇最新一期Anesthesia & Analgesia等SCI杂志的摘要翻译，敬请关注并提出宝贵意见 Circulating miRNAs in acute new-onset atrial fibrillation and their target mRNA network 背景与目的 微小RNA（miRNA）与房颤的发病机制密切相关，并且反映疾病的发生与发展。我们的试验研究观察到新发房颤病人、房颤控制良好的病人及正常窦性心律病人有6种miRNA，而miRNA还可以与mRNA相互作用。 方 法 当急性新发房颤病人（N=5）出现不规则快速颤动节律时，在急诊室采集其血浆。房颤控制良好（N=16）及窦性心律（N=15）病人的血液样本在预约心电图检查后采集。 结 果 RT-PCR分析miR-21, miR-133a, miR-133b, miR-150, miR-328, and miR-499的表达。通过IPA数据库和TargetScan数据库确定了这些miRNA的前30个mRNA靶点，并寻找心血管过程中的miRNA-mRNA相互作用。与房颤控制良好和窦性心律病人相比，急性新房颤中miR-133b(1.4倍)，miR-328 (2.0倍),和 miR-499 (2.3倍)的表达增加。与急性新发AF和窦性心律相比，房颤控制良好病人的miR-21的表达下降了（0.6倍）。miRNA-mRNA相互作用证明SMAD7和FASLG基因是miR-21, miR-133b, 和miR-499的靶点，并且与AF直接相关，参与细胞凋亡和纤维化。 结 论 miRNA的表达不同取决于AF发生条件，急性新发房颤患者的miRNA表达要高于房颤控制良好的病人。miRNA监测可能在临床上评估病人的治疗有效性，从而对房颤早发现早监测，以降低与房颤相关的其他心血管事件风险。 原始文献摘要 Da S A, Jng D A, de Oliveira K M, et al. Circulating miRNAs in acute new-onset atrial fibrillation and their target mRNA network[J]. J Cardiovasc Electrophysiol, 2018. Background: MicroRNAs (miRNAs) are involved in the pathogenesis of atrial fibrillation (AF), acting on development and progression. Our pilot study investigated the expression of six miRNAs and their miRNA-mRNA interactions in patients with acute new-onset AF, well- controlled AF, and normal sinus rhythm (controls). Methods and results: Plasma of acute new-onset AF patients (n=5) was collected in the emergency room when patients presented with irregular and fast-atrial fibrillation rhythm. Samples from well-controlled AF (n=16) and control (n=15) patients were collected during medical appointments following an ECG. Expression of miR-21, miR-133a, miR-133b, miR- 150, miR-328, and miR-499 was analyzed by real-time PCR. Ingenuity Pathway Analysis and the TargetScan database identified the top 30 mRNA targets of these miRNA, seeking the miRNA mRNA interactions in cardiovascular process. Increased expression of miR-133b (1.4-fold), miR-328 (2.0-fold), and miR-499 (2.3-fold) was observed in patients with acute new-onset AF, compared with well-controlled AF and control patients. Decreased expression of miR-21 was seen in patients with well-controlled AF compared to those with acute new- onset AF and controls (0.6-fold). The miRNA-mRNA interaction demonstrated that SMAD7 and FASLG genes were the targets of miR-21, miR-133b, and miR-499 and were directly related to AF, being involved in apoptosis and fibrosis. Conclusion: The miRNAs had different expression profiles dependent on the AF condition, with higher expression in the acute new-onset AF than well-controlled AF. Clinically, this may contribute to an effective assessment for patients, leading to early detection of AF and monitoring to reduce the risk of other serious cardiovascular events. 麻醉学文献进展分享 联系我们 |
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