【原】PI3K通路蛋白在伴有皮质畸形发育的难治性癫痫中的表达

GCTA 2022-06-11 发布于贵州

展开全文

[Expression of PI3K pathway proteins in refractory epilepsy associated with cortical malformation development].

|核心内容：

目的：探讨TSC1、TSC2、p-mTOR、p-4E-BP1、p-p70S6K和p-S6在难治性癫痫相关皮质发育畸形(MCD)组织中的表达。

方法：选择2005-2008年间宣武医院收治的难治性癫痫患者43例，其中局灶性皮质发育不良IIa、IIb型各11例，结节性硬化症10例，胶质瘤11例，其他12例作为对照。

将病例分为7个研究组，采用免疫组织化学EnVision法。

检测各组TSC1、TSC2、p-mTOR、p-4E-BP1、p-p70S6K和p-S6的表达部位和强度。

然后用Image-Pro Plus 6.0图像处理分析软件测量每个样本中阳性细胞的数目、面积、积分吸光度(IA)。统计软件SPSS16。

采用0统计软件对数据进行统计分析。

结果：TSC1和TSC2的免疫定位相似。在畸形神经元、球囊细胞、巨细胞、神经节胶质瘤细胞和正常神经元胞浆中均可见不同程度的表达。正常神经元的TSC1染色明显高于其他标本，而巨细胞的TSC2染色弱于其他标本。P-mTOR主要表达于巨细胞，星形胶质细胞也有表达。

P-4E-BP1表达于球囊细胞、巨细胞和神经节胶质瘤细胞的胞浆和核膜中，巨细胞的染色强度强于球囊细胞，但其染色强度弱于神经节胶质瘤细胞。

P-p70S6K主要表达于巨细胞，在气球细胞中表达较少。

P-S6在所有异常胶质神经细胞中均有表达，而在N-CTX组的正常神经元中几乎不表达。

结论：PI3K通路至少部分参与了MCD的发生，并可能在MCD的发病机制中发挥重要作用。

原文摘要：

OBJECTIVE:To investigate the expression of TSC1, TSC2, p-mTOR, p-4E-BP1, p-p70S6K and p-S6 in refractory epilepsy associated malformation of cortical development (MCD) tissues. METHODS:A total of 43 cases of refractory epilepsy were involved in the study, and all the patients were treated in Xuanwu Hospital during 2005 - 2008, including focal cortical dysplasia type IIa (11 cases) and type IIb (11 cases), tuberous sclerosis complex (10 cases) and ganalioglioma (11 cases), and other 12 cases were used as control. These cases were divided into 7 study groups and immunohistochemical EnVision method was used. To detect the location and intensity of TSC1, TSC2, p-mTOR, p-4E-BP1, p-p70S6K and p-S6 expression in every group. Then the Image-Pro Plus 6.0 image processing and analysis software were used to measure the number, area, integrating absorbance (IA) of positive cells in every samples. The statistical software SPSS 16.0 was used to analyze the data. RESULTS:The immunolocalization of TSC1 and TSC2 was similar. It could be observed the expression of various levels in the cytoplasm of dysmorphic neurons, balloon cells, giant cells, ganglioglioma cells and normal neurons. TSC1 staining in normal neurons was more notably than others but TSC2 staining in giant cells was weaker than other samples. p-mTOR mainly presented in giant cells, which could also be observed in astrocyte. P-4E-BP1 presented in the cytoplasm and nuclear membrane of balloon cells, giant cells and ganglioglioma cells, the staining of giant cells was stronger than balloon cells, but their staining were weaker than ganglioglioma cells. P-p70S6K mainly expressed in giant cells and less commonly presented in balloon cells. P-S6 typically presented in all abnormal glioneuronal cells and it nearly did not present in the normal neurons of N-CTX group. CONCLUSIONS:PI3K pathway, at least in part, involves in the occurrence of MCD, and may play an important role in the pathogenesis.