| gene.list =c('CD3D','CD3E','CD2', 'COL1A1','DCN','C1R', 'LYZ','CD68','TYROBP', 'CD79A','MZB1','MS4A1', 'CLDN5','FLT1','RAMP2', 'CPA3','TPSAB1','TPSB2', 'LILRA4','CXCR3','IRF7') T.cell.marker = c('CD3D','CD3E','CD2') Fib.cell.marker = c('COL1A1','DCN','C1R') Myeioid.cell.marker = c('LYZ','CD68','TYROBP') B.cell.marker= c('CD79A','MZB1','MS4A1') Endothelial.cell.marker= c('CLDN5','FLT1','RAMP2') Mast.cell.marker= c('CPA3','TPSAB1','TPSB2') DC.cell.marker= c('LILRA4','CXCR3','IRF7') gene.list = c('LEF1','TCF7','SELL', 'IL7R','CD40LG','ANXA1','FOS','JUN', 'FOXP3','SAT1','IL2RA','CTLA4', 'PDCD1','CXCL13','CD200','TNFRSF18', 'CCR7','NELL2','CD55','KLF2', 'TOB1', 'GZMK','EOMES','ITM2C', 'GZMH', 'GZMB','LAG3','CCL4L2','CCL5', 'FCGR3A','FGFBP2','TYROBP','CX3CR1', 'AREG','XCL1','KLRC1','GNLY','KLRD1', 'TRDV2','TRGV9','MTRNR2L8', 'TRDV1','KLRC3','CTSW','CD7', 'MK167','STMN1','TUBA1B','HIST1H4C') CD4.naive.marker = c('LEF1','TCF7','SELL') CD4.effector.memory.marker = c('IL7R','CD40LG','ANXA1','FOS','JUN') CD4.Trg.Reg.marker = c('FOXP3','SAT1','IL2RA','CTLA4') CD4.experience.marker = c('PDCD1','CXCL13','CD200','TNFRSF18') CD8.naive.marker = c('CCR7','NELL2','CD55','KLF2') CD8.resident.memory.marker = c('TOB1') CD8.effector.memory.marker = c('GZMK','EOMES','ITM2C') CD8.recently.activate.effector.memory.marker = c('GZMH') CD8.experience.marker = c( 'GZMB','LAG3','CCL4L2','CCL5') NK.cytotoxic.marker = c('FCGR3A','FGFBP2','TYROBP','CX3CR1','GNLY','KLRD1') NK.resting.marker = c('AREG','XCL1','KLRC1','GNLY','KLRD1') Gama.marker = c('TRDV2','TRGV9','MTRNR2L8') Semi.marker = c('TRDV1','KLRC3','CTSW','CD7') Pro.marker = c('MK167','STMN1','TUBA1B','HIST1H4C') # PBMC Marker 包括T细胞（CD3D，CD3E，CD3G，60.00％） CD4 T细胞（CD4，36.94％） - Th1 细胞属于CD4 细胞属，（INF-γ） CD8 T细胞（CD8A , CD8B , 18.53%) 自然杀伤 (NK) 细胞 (NCAM1或CD56，KLRB1，NKG7，5.44％） B细胞（CD19，MS4A1或CD20，CD38，22.27％） 单核细胞（CD14，CD68，FCGR3A或CD16，9.60％） 髓样树突状细胞（mDC的）（CD1C， 0.26%) 浆细胞样树突细胞 (pDCs) ( LILRA4 , 0.18%) 和造血干细胞和祖细胞 (HSPCs) ( CD34 , 0.12%) 红细胞（HBB，0.99%） 巨核细胞（PPBP）, 0.92%) |
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