内皮细胞成管实验 ![]() 备注:本实验为用不同状态(young/aged)的成纤维细胞(FB)的培养基培养内皮细胞,即研究成纤维细胞旁分泌作用对内皮细胞功能的影响,以及Serpin中和抗体(抑制Serpin)在这一过程中的作用。 看图说话:依据上述三要素,我们可以初步描述出:1)相较于young FB组(即年轻的心脏成纤维细胞的培养基),Aged FB组(老年成纤维细胞的条件培养基)促内皮细胞成管能力弱;2)老年成纤维细胞的条件培养液中加入Serpin抑制剂可以逆转老年成纤维细胞对内皮细胞功能的影响,使得内皮细胞成管能力恢复到与年轻的心脏成纤维细胞培养基类似。 原文解析:Inhibition of serpins significantly rescued the
impaired angiogenesis induced by conditioned medium from aged fibroblasts
(Figure 6F) 原图图注:(F)
Tube formation assay of HUVECs that were cultured in conditioned medium
received from young (12 weeks) and aged (20 months) cardiac mouse fibroblasts.
The aged phenotype was rescued by supplementing the aged fibroblast medium with
4 μg of an anti-Serpin antibody. Accumulated tube length was measured in 5
randomly chosen microscopic fields with a computer-assisted microscope using
Axiovision 4.5 (Zeiss) (scale bar: 200 μm; n = 4). ![]() 图2 |
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