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[27]ZHOUX,HUANGW,ZHANGJ,etal.Anovelcom‐770-779.
VerticalHeterogeneityAnalysisofBiochemical
ParametersinOilseedRapeCanopyBasedonFast
ChlorophyllFluorescenceTechnology
1,21,21,2,31,2,3
ZHANGJiafei,WANLiang,HEYong,CENHaiyan
（1.CollegeofBiosystemsEngineeringandFoodScience,ZhejiangUniversity,Hangzhou310058,China;
2.KeyLaboratoryofSpectroscopySensing,MinistryofAgricultureandRuralAffairs,Hangzhou310058,China;
3.StateKeyLaboratoryofModernSpectroscopicInstruments,ZhejiangUniversity,Hangzhou310027,China）
Abstract:Accurateacquisitionofcropcanopybiochemicalinformationisofgreatsignificanceformonitoringcropgrowthand
guidingprecisefertilization.Previousverticaldistributionresearchesofcropbiochemicalinformationweremainlybasedonhy‐
perspectralinversion,whichwaslackoftheassociationofplantphotosynthesisphysiology.Thisstudymainlyinvestigatedthe
verticaldistributioncharacteristicsofbiochemicalparameterssuchaschlorophyll,carotenoid,drymatter,andwatercontentin
theoilseedrapecanopyunderdifferentnitrogentreatmentsatthemid-seedlingstage.Thephotosyntheticperformanceofleaves
wasmeasuredbyusingfastchlorophyllfluorescencetechnology,andlinearregressionandprincipalcomponentanalysiswere
furtherimplementedtoexploretheinternalrelationshipbetweenfluorescenceresponseandbiochemicalparameters.Theresults
showedthat:(1)Thechlorophyllcontent,carotenoidcontent,drymatterandwatercontentoftherapecanopyatthemid-seed‐
lingstageallshowedaparabolicverticaldistribution,whiletheratioofchlorophylltocarotenoidscontentgraduallydecreases
withtheleafpositionandnitrogentreatments,whichwasthesameastheverticaldistributionpatternoffluorescenceparameters
suchasdrivingforcecomprehensiveperformance(DF)andendelectronchainquantumyield(φ)andotherfluorescencepa‐
TotalRo
rameterscouldbeusedtodiagnosenitrogenstress;(2)JIP-testparameters,especiallyDF,hadagoodperformancetoevalu‐
Total
atethechlorophyll/carotenoids,chlorophyllanddrymattercontentofoilseedrapeleaves;(3)Nitrogendeficiencywouldweak‐
enthePSIIandPSIperformanceofoilseedrapeleavesatthemid-seedlingstage,andthemaximumphotochemicalefficiency
(φ)couldbeusedtodiagnosenitrogenstress.TherewasasignificantdifferenceinthePSIperformance,namelyelectrontrans‐
Po
ferefficiencyattheendacceptorsofleavesinthedifferentleafposition,hencethecomprehensiveperformanceparameterDF
Total
couldbeaneffectivecharacterizationoftheverticalheterogeneityofcanopybiochemicalparameters.Thesefindingsindicated
thefeasibilityofapplyingtherapidchlorophyllfluorescencetechnologytocropbiochemicalinformationheterogeneitymonitor‐
ingandprovidednewideasandtechnicalsupportforguidingprecisefertilizationandachievinghigh-qualityandhigh-yield.
Keywords:fastchlorophyllfluorescencetransient;JIP-test;verticalheterogeneity;chlorophyll;carotenoid;nitrogen
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